Planting guide of pineapple tissue culture seedling

2023-01-06

Tissue culture seedling rooting culture

The tissue culture bag is filled with 20-25 ml rooting medium, and after sterilization by high temperature, humid heat, each bag of tissue culture bag is connected to 8-10 tissue culture seedlings, and the tissue culture room is cultured to promote rooting; tissue culture bacteria grow normally And grow to plant height ≥ 13 cm, number of leaves ≥ 8, average leaf length ≥ 12 cm, number of fibrous roots ≥ 10, average root length ≥ 5 cm;

Refine seedlings

Move the tissue cultured seedlings in the tissue culture bag from the tissue culture room to the shade net shed, the shading rate of the shade net shed is 90-95%, and the tissue culture bag is laid flat in the shade net shed for 2 days and the shade net is replaced. For plants with a shading rate of 70-75%, continue to refine the seedlings for 10-15 days. After the leaf color of the tissue cultured seedlings turns dark green, transplant them;

Preparation before transplanting

Preparation of tissue culture seedlings: Take the tissue culture seedlings that meet the transplanting standards from the tissue culture bag, wash the residual medium on the roots with clean water, soak the roots with the medicinal solution, and dry the surface moisture of the tissue culture seedlings under the shade net; Transplanting substrate preparation: set up a border in a shading net shed with a shading rate of 75%, and put the substrate into a nursery cup, the substrate is 1-1. 5 cm above the mouth of the cup; the nursery cup is placed on the border surface, the nursery cup It is advisable to place them without squeezing each other. The day before transplanting, the seedling cup with the substrate shall be soaked with water; the size of the main frame is 5-10 meters × 0.8-1.2 meters × 0.1 meters, and the distance between the two borders is 0. . More than 5 meters, convenient for fertilizer and water management.

Transplant

When transplanting, enclose a hole with a depth of 3 to 5 cm and a width of 2 cm in the center of the soil surface of the seedling cup equipped with the substrate, and put the treated tissue cultured seedlings into the hole to ensure that the stem base of the tissue cultured seedling is 1 cm below the soil surface. Yes, the roots are stretched, down, and not bent; the left hand holds the tissue cultured seedlings, and the right hand gently presses the matrix around the holes to make it firm, 1 plant per cup, pour the root water;

Transplanting period management

After the leaves of the tissue cultured seedlings are dry, spray the medicament immediately for disease prevention and control, and spray the medicament every 7 days; observe the water content of the substrate after transplanting, and pour water once in 1-5 days; wait for new leaves to be drawn Start dressing; 60-70 days after transplanting, increase the light time to make the seedlings fully adapt to the natural light conditions; wait until the seedlings grow to plant height ≥ 25 cm, stem diameter ≥ 2 cm, normal turning green and mature leaves ≥ 20, average When the leaf length is more than 25 cm and the average leaf width (the widest part of the leaf) is more than 2 cm, field planting shall be carried out;

Field planting

The main cause of the garden land after the cultivator and basal fertilizer is applied; the four sides are covered with plastic film, and planting holes are dug on the film and the corresponding side with a plant spacing of 0.3-0.5 meters and a row spacing of 0.4-0.6 meters; the seedling cups for pineapple tissue culture seedlings are removed and kept The soil mass of the root substrate; put the pineapple seedlings together with the substrate soil mass into the planting hole, straighten the seedlings, return to the soil and compact, the soil surface is level with the substrate surface; apply enough root water immediately after planting, and wait for the leaf surface After the water is dry, spray pesticides for pest control, focusing on the heart leaves and stem bases.

Observe the water content of the substrate after transplanting. The number of water spraying depends on the weather conditions. The interval between 1-3 days is appropriate in summer and 3-5 days is appropriate in winter. During continuous rainy weather, shower less carefully; After green, start to spray a mass concentration of 0.1% urea solution and a mass concentration of 0.1% potassium dihydrogen phosphate solution twice a month.

pesticide

The pesticide is a 800-1000-fold dilution of the mass concentration of metalaxyl and chlorothalonil suspension with a mass concentration of 44%, a 800-fold dilution of the mass concentration of 58%·manganese-zinc WP, and a mass concentration of 25% metalaxyl. · 600-800 times dilution of propamocarb wettable powder or 58% metalaxyl by mass concentration · 800-1000 times dilution of manganese-zinc wettable powder, the above-mentioned agents are used in rotation, and the heart leaves and stems should be sprayed when spraying pesticides Base.